Oligonucleotide Synthesis: Methods and Applications (Methods in Molecular Biology)

• ISBN 10: 1588292339
• ISBN 13: 9781588292339
• Edition: 2005
• Release: September 1, 2004
• Format: Hardcover (452 pages)
• List Price: $149.00
• More Details
  
  

  List Price: $149.00

  Tags: Science, Biological Sciences, Biochemistry,

  Book Description:
  A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

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