The Second Edition of Principles of Physical Biochemistry provides the most current look at the theory and techniques used in the study of the physical chemistry of biological and biochemical molecules—including discussion of mass spectrometry and single-molecule methods. As leading experts in biophysical chemistry, these well-known authors offer unique insights and coverage not available elsewhere. Physical techniques currently used by practicing biochemists, including new chapters dedicated to extended material on mass spectrometry and single-molecule methods are included. The book's streamlined organization groups all hydrodynamic methods in Chapter 5 and combines Raman spectroscopy with the spectroscopy section. Relevant problems and applications help readers develop critical-thinking skills that they can apply to real biochemical and biological situations facing professionals in the industry. Biological Macromolecules; Thermodynamics and Biochemistry; Molecular Thermodynamics; Statistical Thermodynamics; Methods for the Separation and Characterization of Macromolecules; X-Ray Diffraction; Scattering From Solutions of Macromolecules; Quantum Mechanics and Spectroscopy; Absorption Spectroscopy; Linear and Circular Dichroism; Emission Spectroscopy; Nuclear Magnetic Resonance Spectroscopy; Macromolecules in Solution: Thermodynamics and Equilibria; Chemical Equilibria Involving Macromolecules; Mass Spectrometry of Macromolecules; Single-Molecule Methods. A useful reference for biochemistry professionals or for anyone interested in learning more about biochemistry.